两种稀土卟啉配合物与大肠杆菌作用的微量热研究

Microcalorimetric Study of the Action of Two Kinds of Lanthanide Monoporphyrinate Complexes on Growth Metabolism of Escherichia Coli

用LKB-2277生物活性检测系统测定了新合成的阳离子型稀土卟啉配合物狖犤Re(TMP)(H2O)3犦Cl,Re=Y、Yb,TMP=5,10,15,20-四(4'-甲氧基苯基)卟啉狚在37℃时对大肠杆菌作用的产热曲线,根据产热曲线求算了在稀土卟啉配合物作用下,大肠杆菌生长代谢的速率常数k,抑制率I,传代时间tG和半抑制浓度cI,50等热动力学参数.结果表明,稀土卟啉配合物在低浓度下对大肠杆菌有刺激作用,高浓度下为抑制作用,犤Yb(TMP)(H2O)3犦Cl的半抑制浓度cI,50为143mg·L-1,其对大肠杆菌的抑制作用优于犤Y(TMP)(H2O)3犦Cl.

The biothermochemical characteristics of the actions of two kinds of cationic lanthanide(III) monoporphyrinate complexes {[Re(TMP) (H2O)(3)] Cl, Re = Y, Yb; TMP = 5, 10, 15, 20 -tetrakis(4'-methoxyphenyl) porphyrin} on growth metabolism of Escherichia coli were studied by means of a LKB-2277 bioactivity monitor at 37 degreesC. The maximum power, P-max; the growth rate constant k(1), k(2); inhibitive ratio I(%) and half inhibition concentration c(I, 50) (mg (.) L-1), respectively, were obtained. Experimental results show that porphyrinate complexes possess the bi-directional biological effection: they stimulate the growth of the E. coli at lower concentration but inhibit the growth at higher concentration. Inhibitory effection of [Yb(TMP) (H2O)(3)]Cl ( c(I, 50) =143 mg (.) L-1) is stronger than that of [Y (TMP) (H2O)(3)] Cl.