溶血对放射免疫测定结果的影响

Influence of Hemolysis on Radioimmunoassay

目的 :了解不同溶血程度对放射测定结果的影响。方法 :配制相同特测物 (FT3、FT4、T3、T4、RT3、CEA、CA5 0、T)浓度的未溶血、轻度 (HGB0 .5 5 g/ L)、中度 (HGB1.10 g/ L)、重度溶血 (HGB2 .2 0 g/ L)标本并对其检测 ,用 t检验比较溶血标本与未溶血标本测定结果的差异性。结果 :除 FT4中度重度、溶血组 P<0 .0 5外 ,其他项目 P>0 .0 5 ,而且 FT4中度、重度溶血组结果与未溶血组比较误差仅为 7.36 %和 5 .6 9%。结论

Objective To observe whether hemolysis have a influence on radioimmunoassay. Methods non hemolytic and light, mediate, serious hemolytic samples with the same concentration for FT3, FT4, T3, T4, RT3, CEA, CA50, T were prepared and determinated. The measured value of non hemolytic samples were compared by the ones of lighe. mediate and serious hemolytic samples respectively. Results There were no significant difference between the results obtained from non hemolytic samples and lighe, mediate, serious hemolytic ones (P>0.05) except for the results of FT4 from mediate, serious ones (P<0.05) and the relative errors between the results of FT4 from non hemolytic and mediate, serious hemolytic samples were not higher, were 7.36 %, 5.69 % respectively. Conclusion The hemolysis has few influence on radioimmunoassay.