化学发光在脱氧核糖核酸探针杂交技术中的应用

Application of Chemiluminescence in Nucleic Acid Hybridization Analysis

本文扼要评述了酶标记及吖啶酯衍生物标记化学发光检测体系在DNA探针杂交技术中的应用及其优缺点,对新近发展起来的金刚烷基取代的1,2-二氧环乙烷衍生物的结构与性能的关系,发光体系的反应机理,影响发光效率的因素,以及如何提高体系的灵敏度等方面进行了较为详细的阐述,意在为读者开展非放射性DNA探针标记物的研究提供方便。

A review with 38 references is given on enzymes and acridinium esters labelled chemiluminescent deoxyribonucleic acid (DNA) probe assays. Applications of horseradish peroxidase, alkaline phosphatase and glucose oxidase labelled chemiluminescent DNA probe are discussed. Details of the most promising recent developed chemiluminescent reagent 1,2-dioxane derivatives are presented.