电针对血管性痴呆大鼠学习记忆能力及脑组织中自由基生成系统的影响

Effects of electroacupuncture on learning and memory and the producing system of free radicals in brain tissue in the rat of vascular dementia

目的:观察电针对实验性血管性痴呆(VD)大鼠学习记忆能力和自由基生成系统的调节作用。方法:选用4—VO模型,用Morris水迷宫测定大鼠学习记忆能力,并检测脑组织一氧化氮(NO)、一氧化氮合酶(NOS)、超氧化物歧化酶(SOD)、丙二醛(MDA)及谷胱甘肽过氧化物酶(GSH-Px)的含量或活性。结果:模型大鼠表现出明显的学习记忆障碍,在定位航行实验中,与假手术组相比,逃避潜伏期显著延长;在空间探索试验中,在原平台象限跨越平台次数明显高于其余3个象限。电针组能显著缩短逃避潜伏期;在原平台象限跨越平台次数显著多于其余3个象限,与假手术组无显著差异。模型大鼠脑组织内NOS活性及NO、MDA含量明显升高,SOD、GSH-Px活性显著降低,与假手术组、电针组及尼莫通组相比均差异显著(P<0.05)或非常显著(P<0.01);这说明电针组与尼莫通组不同程度地降低了脑组织中NOS、NO、MDA含量,提高了SOD、GSH-Px活性。结论,电针对自由基产生与清除的平衡有调控作用,能减轻缺血对脑神经细胞的损害,从而改善VD大鼠学习记忆能力。

Objective: To observe regulative action of electroacupuncture (EA) on learning and memory and the producing system of free radicals in the rat of experimental vascular dementia (VD). Methods: In the 4-VO model rat of vascular dementia (VD), learning and memory were detected by morris's water maze , and nitric oxide (NO), malondiadehyde (MDA) contents, nitric oxide synthase (NOS ), superoxide dismulase (SOD ) and glutathione peroxidase (GSH -Px) activities in the brain were determined. Results: The model rat had obvious learning and memory disorder, and had significantly longer escape latency in the orientation navigation experiment as compared with that of false operation group; and the running over platform times in the original platform quadrant were not significantly higher than other 3 quadrants in the space exploration test. While in the EA group, the escape latency was shortened; the runnig over platform times in the original platform quadrant were significantly more than other 3 quadraunts, with no significant difference to the false operation group. NOS activity, NO and MDA contents in the brain tissue in the model group increased significantly, SOD, GSH-Px activities decreased significantly with significantly differences as compared with that of the false operation group, EA group and Nimotop group (P<0. 05 or P< 0. 01). It is indicated that NO, MDA contents and NOS activity decrease in the EA group and the Nimotop group, and SOD and GSH-Px activities increase in varying degrees in the brain tissue. Conclusion: EA can regulate the balance of production and elimination of free radicals , and can relieve lesion of nervous cells of the brain due to ischemia, so as to improve learning and memory in the rat of VD.