p53基因检测与大肠肿瘤间关系的临床探讨

Clinical Study on the Relationship Between the p53 Gene Examination and Colorectal Neoplasms

目的 探讨p5 3基因检测在大肠癌早期诊断中的临床意义和癌前监控的价值。方法 在内镜下采集 110例大肠肿瘤患者的肿瘤组织标本 ,另以 10例良性肠病的正常肠黏膜组织作对照。采用聚合酶链式反应一单键构象多态性分析 (PCR -SSCP)方法 ,检测p5 3外显子 5～ 8的突变情况 ,同时作病理切片 ,并追踪观察。结果 74例大肠癌中 2 4例p5 3基因突变 ,占 3 2 4% ,其中 17例为中、低分化 ,3例为粘液腺癌 ;3 6例大肠腺瘤中 ,6例p5 3基因突变 ,占 16 7% ,其中 1例Ⅱ～Ⅲ级不典型增生腺瘤患者 10个月后发生癌变。正常肠黏膜组织无 1例p5 3基因突变。结论 p5

Objectives To study the clinical significance of the early diagnosis on colorectal cancer and the value of monitoring precancerous lesions of p53 gene examination. Methods Histological specimens of tumor from 110 patients with colorectal neoplasms were collected under the endoscopy, another 10 normal intestinal mucosa tissues with benign enteropathy as a control group. By PCR-SSCP methods, examined p53 exon 5～8 of mutation circumstance, made pathological section at the same time, and combine to track the observation. Results 24 patients p53 gene mutation were detected out in 78 cases colorectal cancers,counted up 32 4%, among 17 patients were moderate,low differentiation, 3 patients of them were mucinous adenocarcinoma.There are 6 patients p53 gene mutation in 36 cases colorectal adenoma,counted up 16 7%, among them an patient of Ⅱ～Ⅲ class atypical hyperplasia adenoma occurrence canceration in the next 10 months.There was no p53 gene mutation in normal intestinal mucosa tissue.Conclusions p53 gene examination combinated with pathological examination could monitor precancerous lesions and early diagnosis of the colorectal cancer.