人参二醇对四氧化二氮染毒鼠α_1-抗胰蛋白酶及血清酶学变化的影响

Effect of Panaxadiol(人参二醇) on the α_1-antitrypsin and the serum enzyme levels of the mice intoxicated with nitric tetroxide

目的 :探讨人参二醇对四氧化二氮 (N2 O4 )染毒鼠血清蛋白酶抑制物变化及血清酶学变化的影响 ,以寻找简便易行的对 N2 O4 中毒防治的新途径。方法 :12 3只 L ACA雄性小鼠 ,按体质量随机分成对照组、染毒组、染毒加人参二醇组 ,每组 4 1只。中毒后 15 min、1h对照组及染毒组每只小鼠均给予腹腔注射生理盐水0 .2 ml,人参二醇组每只小鼠给予腹腔注射人参二醇 0 .2 ml,连续 7d。观察小鼠的表现和存活情况 ,7d后取眼球静脉血。测定血清α1 抗胰蛋白酶 (α1 AT)及丙氨酸转氨酶 (AL T)、胆碱酯酶 (Ch E)、肌酐 (Cr)、乳酸脱氢酶(L DH )及α羟丁酸脱氢酶 (HBDH )水平。结果 :N2 O4 染毒小鼠伤后 1h血清α1 AT较对照组明显下降 ;人参二醇组小鼠在受伤后 1h血浆α1 AT虽也有不同程度的下降 ,但下降程度明显小于 N2 O4 组。另外 ,人参二醇能够显著降低 N2 O4 染毒小鼠血清 AL T、Cr、L DH及 HBDH水平。结论 :人参二醇能缓解急性肺损伤后血清 α1 AT的下降。在某种程度上能够对抗或缓解急性肺损伤的组织损害。

Objective:To investigate the effect of Panaxadiol(人参二醇) on the α 1antitrypsin (α 1AT) and the serum enzyme levels of the mice intoxicated with nitric tetroxide,and then to find new method to deal with intoxication.Methods:One hundred and twenty three male LACA mice were divided randomly into three groups according to body weight,control group,intoxication group,Panaxadiol group, n=41 .Nitric tetroxide was given to mice through inhalation for 15 minutes.Panaxadiol was administrated by peritoneal injection( 0 2 ml , 7 days ).α 1AT,alanine aminotransferase(ALT),cholinesterase(ChE),creatinine(Cr),lactate dehydrogenase(LDH) and αhydroxybutyrate dehydrogenase(HBDH) were detected after intoxication 1,12,48 hours respectively.Results:Compared with control group,intoxication of nitric tetroxide could reduce the α 1AT level significantly.Administration of Panaxadiol could not affect the normal α 1AT level of the control group but could ameliorate the decreased level of α 1AT of the intoxication group.Furthermore, administration of Panaxadiol could attenuate the deterioration of ALT?Cr?LDH and HBDH respectively. Conclusion: Panaxadiol can reduce the increasing level of α 1AT,ALT,Cr,LDH and HBDH which induced by nitric tetroxide. These beneficial effects of Panaxadiol may be due,in part,to the direct inhibitory effect on neutrophil elastase,in addition to protect the dysfunction of liver and kidney.