大剂量维生素C对肠黏膜屏障的保护作用

Protective effects of Vitamin C on intestinal mucosal barrier of the scalded rats

目的 研究大剂量维生素C对烫伤后大鼠肠黏膜屏障的保护作用。方法 选用大鼠烫伤模型 ,烫伤面积为30 % ,深度为Ⅲ度。大鼠随机分为 4组 ,即烫伤组、模拟烫伤组、维生素C治疗组和安慰剂治疗组。观察维生素C对烫伤大鼠肠道细菌易位、血浆内毒素水平和肠黏膜内超氧化物歧化酶 (SOD)的影响。结果 严重烫伤可致肠黏膜屏障破坏 ,肠道内细菌易位至肝、脾、肠系膜淋巴结 ;维生素C可降低细菌易位的发生率和易位量 ,与烫伤组有显著差异 (P <0 0 5 ) ;且烫伤可致血浆内毒素水平明显升高 ,尤以烫伤后 10h显著 ,而 2 4h后有所下降 ;维生素C可明显抑制肠道内内毒素的吸收 (P <0 0 1) ;此外 ,维生素C对烫伤后肠黏膜内氧自由基有明显的清除作用。结论 维生素C对烫伤大鼠肠黏膜屏障有明显的保护作用 ,能抑制肠道细菌易位和肠道内内毒素吸收。

Objectives To study the protective effects of Vitamin C on intestinal mucosal barrier of the scalded rats.Methods The scalded rats′ model was used.The rats were randomly divided into four groups, scald group, sham-scald group, Vitamin C treatment group, and placebo treatment group.The bacterial translocation, the level of plasma endotoxin and the amount of tissue superoxide dismutase(SOD) were detected in above groups.Results The bacteria in gut translocated into livers, spleens and mesenteric lymphatic nodes after thermal injury.Moreover, the number of bacteria colonized in those organs were very larger.However, Vitamin C could obviously inhibit bacterial translocation from the gut.There was significant difference between the scald group and Vitamin C treatment group (P<0.05). Furthermore, Vitamin C could also decrease the level of plasma endotoxin and reduce the consumption of SOD in livers, guts and serum (P<0.01 vs scald group).Conclusion Vitamin C had a protective function for intestinal mucosal barrier of the scalded rats.