X-刀治疗大鼠C6脑胶质瘤诱发的细胞凋亡

Apoptosis induced by X-knife treatment of C6 glioma in rat

目的 研究X 刀治疗胶质瘤的作用 ,并探讨诱发细胞凋亡效应。方法 采用DNA琼脂糖凝胶电泳、超微结构观察及新兴的DNA片段末端标记方法对各组C6大鼠脑胶质瘤发生的细胞凋亡进行研究。结果 1 5、 2 0及 30Gy各组在治疗后 2 4h内均出现明显的细胞凋亡 ,DNA末端标记法检测的结果不但与前述方法结果相符 ,还显示出其准确性高等特性。研究中还发现各治疗组均有坏死同时出现。结论 X 刀治疗后诱发细胞产生凋亡的同时还致细胞坏死 ,这种效应随剂量增加渐趋明显 。

Objective We used the C6 rat glioma model to evaluate the potential role of linear accelerator radiosurgery (X knife) for glial neoplasms. Methods SD rats were performed enhancement CT examination after implantation of C6 glioma cells into the right frontal region to confirm the onset of tumor and select appropriate one for radiosurgery. 46 rats were randomized to control or treatment groups, 32 underwent stereotactic radiosurgical (SRS) treatment using the 5 mm collimator of the X knife. Animals were killed at 4 hour, 1 day, 1 week or premortem following 15, 20, 30 or 40 Gy X knife radiosurgery. Results We found out some evidences of apoptosis in this study. Microscopic analysis results showed that 15, 20 or 30 Gy groups displayed significantly apoptosis at 4 hour and 1 day after radiosurgery treatment compared to control and 40 Gy groups (P<0 0001). This result was correlated completely to ultrastructural observation and DNA agarose gel electrophoresis which showed the 'ladder' pattern clearly within 1 day after SRS treatment in 15, 20 or 30 Gy groups whereas the 40 Gy group was vaguely. The 40Gy group showed more necrosis than apoptosis. The results of in situ end labeling detection of apoptotic cells also received identical outcome with the methods mentioned above.Conclusions the apoptotic mode of cell death and necrosis may represent the response in this X knife treated rat C6 glioma model.