应用荧光定量PCR检测血清中HCV RNA

Quantitation of HCV RNA of HCV antibody positive serum samples in Nanjing

目的 :探讨荧光定量PCR(FQ PCR)法检测血清中丙型肝炎病毒 (HCV)含量的敏感性、特异性。方法 :采用FQ PCR和逆转录巢式PCR同步检测 76份HCV抗体阳性血清标本 ,并对两种方法的检测结果进行对照比较。结果 :FQ PCR定量HCVRNA拷贝数在 10 2 ～10 6拷贝·μl-1,其中低于 10 3 拷贝·μl-1占 2 0 .93 % ,10 3 ～ 10 5拷贝·μl-1占 60 .47% ,高于 10 5拷贝·μl-1占 18.60 %。 76份HCV抗体阳性血清中 ,巢式PCR检测阳性为 45份 ,FQ PCR检测阳性为 43份 ,二者相对符合率为 97.3 7%。结论 :FQ PCR检测HCVRNA与常规定性巢式PCR特异性、敏感性基本一致。

Objective To explore the sensitivity and the specificity of fluorescence quantitative PCR(FQ-PCR) by which HCV RNA level of serum is determined.Methods HCV RNA in serum was determined by RT nested PCR and FQ?PCR simultaneously.Results The copies of HCV RNA ranged from 10 2 copies·μl -1 to 10 6 copies·μl -1 ,among which,20.93% was below 10 3 copies·μl -1 ,60.47% was between 10 3 copies·μl -1 and 10 5 copies·μl -1 ,the rest was more than 10 5 copies·μl -1 .In 76 antibody positive samples,45(60.8%) was nested PCR positive,43(58.1%) was FQ?PCR positive,showing no statistical difference.Conclusions The level and distribution of HCV RNA in Nanjing is the same as in other places reported in literature.FQ?PCR method has the same sensitivity and specificity as RT?nested?PCR.