低剂量碘化钾治疗大鼠实验性乳腺纤维囊性增生

Experimental therapy of fibrocystic breast disease by low dose of potassium iodide in rats

目的 :观察低剂量碘化钾对大鼠乳腺纤维囊性增生的防治作用和对血雌激素、孕激素分泌的影响。方法 :采用SD雌大鼠复制乳腺纤维囊性增生模型 ,低剂量碘化钾灌胃或在造模同时预防性灌胃。放射免疫方法测定给药前后血雌激素、孕激素和黄体生成素水平 ;用打分法评定用药前后鼠乳腺病理学改变 ,并与他莫昔芬的作用进行比较。结果 :模型鼠给予碘化钾可减轻乳腺纤维囊性增生的病理改变 ,剂量依赖性地降低血雌二醇和雌三醇的水平 ,升高血孕酮和黄体生成素的水平 ,预防性给低剂量碘化钾一定程度抑制了乳腺纤维囊性增生。结论

Objective To investigate the effect of low dose of potassium iodide(KI) on fibrocystic breast disease (FBD) in modeling SD rats.Method The fibrocystic breast disease model was established by subcutaneous injection of estradiol benzoas 0.5?mg·(kg·d) -1 for 25 days,followed by progesterone 4?mg·(kg·d) -1 for 5 days in female SD rats.After the model was established, the rats were divided into three groups:the model control group (treated by NS),the KI therapy groups (treated by KI 3,9 or 27?mg·(kg·d) -1 and tamoxifen group (TAM)(treated by TAM 12?mg·(kg·d) -1 ).The preventive therapy with KI 9?mg·(kg·d) -1 was performed since the beginning of modeling.The therapeutic effect of KI was evaluated by pathological examination,and the serum levels of estradiol(E 2),estriol(E 3),progesterone and luteinizing hormone(LH) were determined by radioimmunoassay method.Results After administering KI 3,9 or 27?mg·(kg·d) -1 30?d,the pathological changes of the modeling rats were significantly abated.The serum levels of E 2 and E 3 were decreased in a dose dependent way,whereas,the serum levels of progesterone and LH were significantly higher than those of model control rats.The production and development of fibrocystic breast were attenuated by preventive administration of KI in rats.Conclusion KI might be used for therapy and prevention of fibrocystic breast disease.