聚合酶链反应和DNA探针杂交法检测沙眼衣原体的比较

Polymerase chain reaction vs DNA probe hybridization for detecting Chlamydia trachomatis in female urogenital tract

目的 :探讨聚合酶链反应 (PCR)和DNA探针杂交法检测泌尿生殖道沙眼衣原体 (Ct)的临床诊断价值。方法 :应用这两种方法对未使用过抗衣原体药物的 80例宫颈炎患者同时进行Ct检测。结果 :PCR法检出Ct基因的阳性率 (2 3 .8% )高于DNA探针杂交法 (15 .0 % ) ,两者比较有显著性差异。结论 :PCR法检测Ct较DNA探针杂交法敏感 。

Objective To explore the clinical diagonstic value of polymerase chain reaction (PCR) and DNA probe hybridization for detecting Chlamydia trachomatis (Ct) infection of urogenital tract.Method 80 specimens from cervicitis subject were examined by PCR and DNA probe hybridization simultaneously.Result The positive rate of PCR (23.8%) was higher than that of DNA probe hybridization (15.0%) significantly.Conclusion PCR is more sensitive than DNA probe hybridization in detecting Ct infection,and the combination of two would be more preferable.