P糖蛋白及p53基因蛋白在骨肉瘤组织中的表达及相关性

Relationship between expressions of P-glycoprotein and p53 protein in osteosarcoma

目的 探讨多药耐药及 p5 3基因蛋白在骨肉瘤组织中的表达及其相关性 ,以及对骨肉瘤预后的影响。方法 应用免疫组化方法 (S P法 )测定由多药耐药基因介导的P糖蛋白 (Pgp)及 p5 3基因蛋白在 33例骨肉瘤组织中的表达。男 2 4例 ,女 9例 ,年龄平均 2 0 .6岁。ⅡA5例 ,ⅡB2 3例 ,ⅢB5例。结果 19例获得平均 1年 7个月随访 ,复发 3例 ,肺转移 11例 (其中 6例死亡 )。P糖蛋白阳性表达率为 4 5 .5 % ,p5 3基因蛋白阳性表达率为 5 7.6 % ,两者均与预后有关 ;P糖蛋白与 p5 3蛋白在骨肉瘤细胞中表达无相关性。结论 Pgp表达是骨肉瘤产生多药耐药的重要因素 ,Pgp及 p5 3基因蛋白同时表达的患者预后更差。p5 3基因是否参与或介导骨肉瘤多药耐药的发生尚有待于进一步研究和探讨。

Objective To study the expression of P glycoprotein in osteosarcoma and the correlation with the expression of p53 gene. Methods The P glycoprotein and p53 expressions of 33 osteosarcomas were detected by immuno histochemistry (S P) method.There were 24 in males,9 in females and with the mean age of 20.6 years, 5 inⅡ A , 23 inⅡ B and 5 inⅢ B .Results 9 patients were followed up with the average time of 1 year and 7 months, 3 recurred and 11 metastated to the lungs and 6 died. the positive expression rate of p glycoprotein and p53 were 45.5% and 57.6%,respectively.Both related with the prognosis of osteosarcoma, there was no relationship between the expressions of P glycoprotein and p53 gene. Conclusion The expression of MDR induced by P glycoprotion was an important factor of multidrug resistance in osteosarcoma,and affected the prognosis, patients with co expression of P glycoprotein and p53 had poorer prognosis.It remains to be proved whether the multidrug resistance is induced by p53 gene in osteosarcoma.