bcl-2和Fas/Apo-1基因在急性白血病细胞中的表达及其意义的探讨

The studies on the expression of bcl-2 and Fas/Apo-1 genes in acute leukemia

目的 :研究 bcl- 2和 Fas/ Apo- 1基因在急性白血病 (AL)细胞中表达及其与 AL 的分型、临床特征、疗效及预后因素的关系。方法 :采用免疫组化 SP法检测 5 4例初治 AL骨髓细胞的 bcl- 2和 Fas/ Apo- 1基因蛋白的表达情况。结果 :AL骨髓细胞中 bcl- 2基因的表达显著高于正常对照组 (P <0 .0 5 ) ,而 Fas/ Apo- 1基因的表达低于正常对照组 (P <0 .0 5 )。 bcl-2和 Fas/ Apo- 1基因表达在急性髓系白血病 (AML)与急性淋巴细胞白血病 (AL L)无显著差异 ;在 AML 的亚型中 ,bcl-2在 M4及 M5中的表达高于 M1、M2和 M3(P<0 .0 5 ) ;而 Fas/ Apo- 1的表达在各亚型中无显著性差异。bcl- 2的表达与初诊时的白细胞数呈正相关 (r=0 .4 4 ,P <0 .0 5 ) ,与疗效呈负相关 (r=- 0 .4 0 ,P <0 .0 5 ) ;急性淋巴细胞白血病中 Fas/ Apo-1的表达与疗效呈正相关 (r=0 .34,P <0 .0 5 ) ,与骨髓原始细胞数呈负相关 (r=- 0 .71P <0 .0 5 )。结论 :bcl- 2和 Fas/ Apo-1基因的紊乱是 AL 形成与发展的原因之一 ,且与 AL 的某些临床特征。

Objective:To study the expression of bcl 2,Fas/Apo 1 genes in acute leukemia and their significance in the FAB classification,some clinic features,treatment outcome and prognostic factors of acute leukemia(AL) Methods:By using immunohistochemical staining method analysis,the expressions of bcl 2 and Fas/Apo 1 genes in 54 cases of untreated AL were detected Results:Overexpressions of bcl 2 genes and low expressions of Fas/Apo 1 gene (P<0 05) were found in AL (P<0 05).The expression of bcl 2 and Fas/Apo 1 in acute myeloid leukemia (AML) and acute lymphoid leukemia (ALL) had no significance.According to FAB classification,the expression of bcl 2 in type M4 and M5 was higher than that in M1,M2 and M3 (P<0 05).the expression of bcl 2 gene was positively correlated with the WBC number(r=0 44,P<0 05) and was negatively correlated with the treatment outcome (r=-0 40,P<0 05).The expression of Fas/Apo 1 in various types had no significance (P>0 05).There was positively correlation of Fas/Apo 1 expression with the treatment outcome (r=0 34,P<0 05) and negatively correlation with percentage of blast cells in bone marrow (r=-0 71,P<0 05) Conclusion:Abnormal expressions of bcl 2 and Fas/Apo 1 genes in AL play a pivotal role in pathogenesis and progression of AL.These apoptosis regulating genes were still related to some clinic features,treatment outcome and prognostic factors of leukemia