高LET α粒子体外照射诱发中国仓鼠肺细胞的恶性转化

NEOPLASTIC TRANSFORMATION INDUCED BY Pu α PARTICLES IN CHINESE HAMSTER LUNG CELLS

本文介绍了^(238)pu α粒子体外照射诱发中国仓鼠肺(CHL)细胞恶性转化的特征。初步结果表明,在0.5—2.5 Gy的剂量范围内,CHL 细胞的剂量-存活曲线符合单次击中单靶模型:存活分数 S=exp(—D/D_0),平均致死剂量D_0=0.515±0.014 Gy。高 LET α粒子照射后的早期(第2代),CHL 细胞增殖能力明显受到抑制,传至第20代时,细胞增殖能力则增强;观察到某些生物学的转化特征,并呈显一定的剂量效应关系。将已发生转化的 CHL 细胞接种到免疫缺陷型受体动物体内后,4—5周均形成肿瘤。与成年大鼠肺成纤维细胞相比较,CHL 细胞对高 LET α粒子的照射具有一定的耐受性。

The Chinese Hamster Lung(CHL)cells were served as a target one for investigatingtransformation induced by 5.25 MeV a particles from a specially constructed Pu source.At doseranges of 0.5—2.5 Gy,the dose-survival curve was fitted to a single target model:S=exp(—D/D_0),leather dose D_0=0.515±0.014 Gy.The growth of CHL cells were greatly depressed in earlystage(passage 2)after irradiation,but were obviously enhanced with increasing of passaging(up topassage 20).Some of neoplastic features were observed,and they have a dependence on the dose ofirradiation.Finally,tumors were developed 4-5 weeks after injecting the CHL cell populationssubtaneously into the back of BALB/c nude.In comparision with lung fibroblasts in adult rats,theCHL cells are of resistence to high LET α particles.