血小板糖蛋白受体ⅠaC807T基因多态性与心肌梗死关系的研究

A study of the relationship between platelet glycoprotein receptorⅠa C807T gene polyniorphism and myocardial infarction

目的 :检测中国人群血小板糖蛋白 (GP)受体 a C80 7T基因多态性 ,以明确中国人群血小板 GP a C80 7T的基因型 ,并探讨其多态性与心肌梗死 (MI)之间的关系。方法 :提取人基因组 DNA,应用引物序列特异性多聚酶链反应 (SSP- PCR)技术对小于 6 0岁的 MI患者 72例和对照者 70例分别进行血小板 GP受体 a C80 7T基因多态性检测。结果 :中国人群血小板 GP a C80 7T具 3种基因型 (TC,CC,TT) ,MI患者 T等位基因携带频率明显高于对照组 (6 4 % vs4 7% ,P<0 .0 5 )。结论 :年龄小于 6 0岁的 MI患者 T等位基因携带频率高 ,血小板膜 GP a受体的遗传性差异在

AIM:To detect the platelet glycoprotein(GP) receptor Ⅰa C807T gene polymorphism of Chinese,and to investigate the possible relationship between myocardial infarction(MI) and platelet GP Ⅰa C807T gene polymorphism. METHODS:Leukocyte DNA was isolated from whole blood using standard procedures. Platelet GP receptor Ⅰa C807T gene polymorphism was detected by sequence specific primer(SSP) polymerase chain reaction (PCR) method in 72 patients with MI(<60 years old) and 70 control subjects. RESULTS:The platelet GPⅠa of Chinese has three gene types(TC,CC,TT). The frequency of T allele was higher in patients with MI than that in control subjects(64% vs 47%, P <0.05)?CONCLUSION:The frequency of T allele was significantly increased in patients with MI(<60 years old). Inherited platelet GP Ⅰa variations might play an important role in MI.