钼蓝光度法测定头孢氨苄

Spectrophtometric Determination of Cefalexin by Molybdenum Blue

研究了头孢氨苄的降解产物与钼多酸在低浓度硫酸介质中形成钼蓝的反应 .建立了硫酸浓度为 0 .0 2mol/L,钼多酸浓度 1.6× 10 - 3m ol/L,水浴加热 4 0 min,测量波长为 6 6 0 nm条件下头孢氨苄的测量方法 .结果表明 :头孢氨苄含量在 0～ 4 5 m g/L 范围内符合比尔定律 ,对 10 mg/L 头孢氨苄测定 7次的相对标准偏差为 2 .0 % ,加标回收率为 95 %～ 10 5 % .本法线性范围宽 ,稳定性好 .

A new method for the spectrophtometric determination of cefalexin was proposed. In 0.02 mol/L H 2SO 4, molybdenum acid was reduced by cefalexin and formed .The maximum absorption of the reaction product, is at 660nm,Beer′s law is obeyed inside the concentration range of 0～45 mg/L .Recoveries of standard addition were 95%～105%.The relative standard deviation for the determination of 10 mg/L cefalexin is 2.0%( n =7).