原发性肝细胞癌细胞增殖、凋亡及P16表达和肿瘤分化程度关系的研究

The study on the relation between cell proliferation and apoptosis and P16 in tumour differentiation in primary hepatocellular carcinoma

目的 :探讨原发性肝细胞癌 (HCC)中细胞增殖、凋亡及P16的表达与肿瘤分化程度的关系。方法 :采用免疫组化法对 5 0例HCC患者 (HBsAg均阳性 )癌细胞增殖细胞核抗原Ki 6 7和P16的表达进行了定位和半定量分析 ,用Tunel法对细胞凋亡进行检测 ,对结果进行统计学分析。结果 :HCC细胞分化程度越差 ,细胞增殖能力越强 ,凋亡细胞越少 ,P16和肿瘤分化程度负相关。结论 :HCC细胞中Ki 6 7和凋亡及其调控基因P16与HCC的分化程度有关 ;HCC细胞中增殖 凋亡失调 ,是导致HCC发生发展的重要原因 ;P16的表达与细胞增殖和凋亡相关 ,通过导入野生型P16诱导HCC中细胞凋亡或抑制其增殖 。

Objective:To study the relation between cell proliferation,apoptosis and the expression of P16 and tumour differentiation in primary hepatocellular carcinoma (HCC). Methods:The study was performed on 50 paraffin fixed hepatic tissue which were randomly collected from HCC patients.By immunohistochemistry methods,Ki 67 and P16 expression were analyzed.Tunel method was adoped to analyze the apoptosis occurrence.x 2 text and correlation analysis were adopted for statistical analysis.Results:Proliferation/apoptosis ratio has a positive correlation to HCC cell differenation,P16 appears in cell nucleus.Conclusion:The positive relation between Ki 67 expression and apoptosis exists.Proliferation and apoptosis can affect HCC in growth and differentiation of HCC.P16 is related to cell proliferation and apoptosis.Inducing apoptosis and inhabit cell proliferation can improve the clinical therapy effects of HCC.