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表皮生长因子对人肝癌细胞EGFR/DNA表达的影响 被引量：1

Effects of Epidermal Growth Factor on the Expression of EGFR/DNAof Human Hepatoma Cells
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摘要 目的 探讨表皮生长因子（EGF）对人肝癌细胞EGFR/DNA表达的影响。方法 采用双参数流式细胞免疫荧光技术检测,结果　EGF短时间内上调肝癌细胞EGFR及DNA表达,随时间延长表现出下调作用。两种相对立的作用呈时间依赖性。结论 EGFR表达的变化可能是EGF对肝癌细胞表现出两种相对立作用的机理之一。 Objective To assay the effects of epidermal growth factor (EGF) on the expression of EGFR/DNA of human hepatoma cells. Methods Bivariated cellular immunofluorescence technique was used. Results EGF increased the expression of EGFR/DNA within a short time, and then decreased the expression of EGFR/DNA. It had two kinds of opposite effects on the growth of hepatoma cells, showing a time - dependant relationship. Conclusion The expression change of EGFR is probably one of the mechanisms of EGF efficiency on the growth of hepatoma cells.
作者 金志明 陈心江 刘涛 王天翔 朱上林
机构地区 上海第二医科大学瑞金医院卢湾分院普外科 瑞金医院普外科
出处 《上海第二医科大学学报》 CSCD 2002年第6期489-491,共3页 Acta Universitatis Medicinalis Secondae Shanghai
基金 国家自然科学基金资助项目(39470677)
关键词 表皮生长因子 双参数流式细胞免疫荧光技术 肝癌细胞 DNA表达 EGFR表达 epidermal growth factor bivariated cellular immunofluorescence technique hepatoma cells
分类号 R735.7 [医药卫生—肿瘤]
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参考文献10

  • 1Huang S,Trujillo JM, Chakrabarty S. Proliferation of human colon cancer cells: role of epidermal growth factor and transforming growth factor α[J]. IntJCancer, 1992.52(6) :978 -986.
  • 2Reeves JR, Richards RC, Cooke T. The effects of intracolonic EGF on mucosal growth and experimental carcinogenesis[ J ]. Br J Cancer,1991,63(2) :223 -226.
  • 3Vambutas A, Lorenz TP, Steinberg BM. Laryneal papilloma cells have high levels of epidermal growth factor receptor and respond to epidermal growth factor by a decrease in epithelial differentiation [ J ]. Cancer Res, 1993,53(4) :910 -914.
  • 4Yade K. Effect of intracellular pH and two growth factors, epidermal growth factor and human hepatocyte factor, on DNA synthesis in nonregenerating and regenerating hepatocytes and hepatoma cells [J].Osake City Med J, 1994,40(2) :53 -69.
  • 5Murayama Y. Growth - inhibitory effects of epidermal growth factor on human breast cancer and carcinoma of the esphagus transplanted into nude mice [J]. Ann Surg, 1990, 211 (3): 263-268.
  • 6Kamata N, Chida K, Rikimaru K, et al. Growth - inhibitory effects of EGF and overexpression of its receptors on human squamous cell carcinomas in culture[J]. Cancer Res, 1986,46:1648 - 1653.
  • 7Briamd P, Nielsen KV, Madsen MW, et al. Trisomy 7p and malignant transformation of human breast epithelial cells following epidermal growth factor withdrawal[J]. Cancer Res, 1996,56(3 ) :2039-2044.
  • 8Mansbridge JN,Ausserer WA, Knapp MA, et al. Adaptation of EGF receptor signal transduction to three - dimensional culture condition:changes in surface receptor expression and protein tyrosine phosphorylation[J]. J Cell Physiol, 1994,161:374 -382.
  • 9Dangles V, Femema F,Laine V, et al. Two- and three -dimensional cell structures govern epidermal growth factor survival function in human bladder carcinoma cell lines[ J ]. Cancer Res, 1997,57 (8):3360 - 3364.
  • 10金志明,王天翔,朱上林.不同作用时间内表皮生长因子对肝癌细胞凋亡的影响[J].外科理论与实践,2001,6(2):116-118. 被引量：1

二级参考文献4

  • 1[1]Reddy Avula CP, Muthukumar A, Fernandes G. Calorie restriction increases Fas/Fas-ligand expression and apoptosis in murine splenic lymphocytes. FEBS Lett. 1999 Sep 17, 458: 231.
  • 2[2]Holt PR, Moss SF, Heydari AR, et al. Diet restriction increases apoptosis in the gut of aging rats.J Gerontol A Biol Sci Med Sci. 1998, 53: B168.
  • 3[3]Briand P, Nielsen KV, Madsen MW, et al. Trisomy 7p and malignant transformation of human breast epithelial cells following growth factor withdrawal. Cancer research, 1996, 56: 2039.
  • 4[4]Dangles V, Femema F, Laine V, et al. Two-and three dimensional cell structures govern epidermal growth factor survival function in human bladder carcinoma cell lines. Cancer reseach, 1997, 57: 3360.

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上海第二医科大学学报
2002年 第6期

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