摘要
采集健康的和感染香蕉花叶心腐病毒 (CMV B)的香蕉吸芽 ,进行常规组织培养 ,用间接ELISA法对吸芽叶片及各代不定芽进行病毒检测。根据检测结果并结合继代苗和生根苗的症状表现 ,发现CMV B在常规的组培繁殖中是逐代传递的 ;在 2 8± 2℃和较弱的室内自然散射光条件下培养 ,各代苗病毒检测灵敏度基本一致 ;高温和强光照培养影响病毒的增殖。研究结果还表明 ,在同一带毒母株的不同吸芽和同一带毒吸芽组培繁殖的后代中 ,病毒分布不均匀 ;各代生根苗显症率不一致 ,假植后显症率提高 ;
Healthy and virus-infected suckers were used as explants in banana tissue culture. The results detected by indirect ELISA showed that virus(CMV-B )could be transferred from initial to later multiplicating generations. It kept almost the same sensitivity of detection at different generations when the samples were grew at 28±2℃ and in natural random light. Virus replication would be affected by high temperature and illumination. Virus distribution is irregular in the clones from either the same sucker or the same main stem.The rate of the plantlets which shown symptoms is different in different generations and increased after temporary planting. There was no relation between symptom expression and virus concentration.
出处
《中国南方果树》
北大核心
2002年第6期35-39,共5页
South China Fruits
基金
广西农业科学院科技发展基金资助项目 (2 0 0 0 17)
