当归对兔肾缺血再灌注损伤的影响

EFFECTS OF RADIX ANGELICAE SINENSIS ON THE ACTIVITY OF ATPASE AND THE AMOUNTS OF BASIC FIBROBLAST GROWTH FACTOR AFTER KIDNEY ISCHEMIA AND REPERFUSION IN RABBITS

目的 :研究当归对兔肾缺血再灌注损伤的防治作用及其机制。方法 :将 2 5只健康成年日本大耳白兔 ,随机分为手术对照组、单纯肾缺血再灌注 (IR)组和当归注射液 (RAS)处理IR(RAS +IR)组。肾缺血 1h再灌注48h时 ,取肾作电镜检查 ;测血清肌酐 (Cr)、肾组织中ATP酶活性和碱性成纤维细胞生长因子 (bFGF)的含量。结果 :IR组肾组织变性改变显著 ,RAS +IR组病变轻微。同对照组相比 ,IR组血清Cr含量升高 (P <0 .0 5 ) ,肾中ATP酶活性和bFGF含量降低 (P <0 .0 5和P <0 .0 1) ;RAS +IR组较IR组血清Cr含量降低 (P <0 .0 5 ) ,肾中ATP酶活性升高(P <0 .0 5 ) ,与对照组差异无显著性 (P >0 .0 5 ) ;RAS +IR组肾中bFGF含量较IR组和对照组均升高 (P <0 .0 1和P<0 .0 5 )。结论 :当归可能通过保护ATP酶、诱bFGF产生而减轻IR肾损伤。

Objective: To study the effects of Radix Angelica Sinensis(RAS) on renal ischemia reperfusion in rabbits and to explore its mechanism.Methods: Twenty-five rabbits were divided randomly into the operative control group(control group) ,renal ischemia reperfusion injury group(IR group )and RAS-treated group(RAS+IR group). At reperfusion 48hours after renal ischemia for 1hour, the renal tissue was detected by electron-microscopy and the amounts of creatinine(Cr) in serum , the activity of ATPase and the amounts of bFGF in renal tissue were determined.Results: The degenerative changes were remarkable under electronmicroscope in renal tissue in IR group, but the changes in RAS+IR group were slight. Compared with control group,the amounts of Cr in IRgroup were signicantly increased(P<0.05), the activity of ATPase and the amounts of bFGF in renal tissue was markedly decreased (P<0.05,P< 0.01 );Compared with IR group, the amounts of Cr in RAS+IR group were signicantly decreased(P<0.05), the activity of ATPase in renal tissue were markedly increased (P<0.05);There were no signifcant differences between the control group and RAS+IR group(P>0.05); while the amount of bFGF in RAS+IR group increased compared with IR group and control group(P<0.01,P<0.05). Conclusion:RAS can alleviate the renal ischemia reperfusion injury by protecting the activity of ATPase and inducing the production of bFGF.