古尼虫草的生物活性物质I.含肽镇痛组分的分离及性质

Bioactive Substances from Cordyceps gunnii I.Separation, Purification and Properties of Analgesic Substance

用理化分离分析和生物检测方法相结合 ,从古尼虫草 (Cordycepsgunnii(Berk .)Berk .)无性型 ,古尼拟青霉 (PaecilomycesgunniiLiang)菌丝体中初步分离纯化得到镇痛物质 ,该物质经氨基酸组成分析表明是一种酸性氨基酸残基高的肽类物质。经不同温度、pH及蛋白酶的稳定性试验分析观察到这种肽类物质对酸稳定、在酸性条件下抗热 ,对胃蛋白酶、胰蛋白酶部分敏感 ,对蛋白酶K不敏感。经小鼠竖尾法和大鼠攻击法测定 。

The analgesic substance from mycelia of Paecilomyces gunnii Liang that is anamorph of Cordyceps gunnii(Berk.)Berk. was isolated and purified by combined with separation and analyzing and biology experiment. Based on the results by amino acid analyzer showed that the analgesic substance from P.gunnii is not dissociate amino acid but a bioactive peptide consisting of more acidic amino acids .The analgesic bioactive substance was stable to acids and host under acidic condition. It was not sensitive to proteinase K and partly sensitive to pepsin and trypsin. By animal tests of Mus musculus and Rattus norxegius showed the analgesic bioactive substance have no drug addiction being similar to morphia.