p38MAPK抑制剂SB-202190对大鼠胶质瘤C6细胞的双重作用

Specific inhibitor of p38MAPK SB-202190 have dual effects on rat glioma C6 cells

目的 探讨 p38MAPK抑制剂SB 2 0 2 190对C6细胞生物活性的影响。方法 采用透射电镜的方法和DNA琼脂糖凝胶电泳的方法观察SB 2 0 2 190诱导胶质瘤细胞的凋亡 ;应用流式细胞仪检测细胞周期变化和是否有凋亡发生。结果 浓度分别为 10、2 0、4 0 μmol·L-1的SB 2 0 2 190能促进C6细胞的细胞周期从G1期过度到S期 ;但 6 0 μmol·L-1的SB 2 0 2 190却诱导C6细胞凋亡 ,凋亡率与时间呈正相关 ,透射电镜观察结果显示 ,凋亡细胞的体积缩小、细胞核浓聚缩小 ,染色质固缩聚集于核膜下 ,胞质浓缩 ,有的细胞染色质浓缩至核膜下成新月状 ;琼脂糖凝胶电泳呈现出特征性的DNA“梯状”带。结论 SB 2 0 2 190在低浓度时促进C6细胞增殖 ,高浓度时诱导细胞凋亡。

AIM To study the effect of the specific inhibitor of p38MAPK,SB 202190, on the biological characteristics of glioma C6 cells. METHODS The morphological and adhesive changes of the cells were observed under an inverted microscope and C6 cells undergoing apoptosis were determineed by electron microscope and agrose gel electrophoresis after treatment of SB 202190. Flow Cytometry was adopted to measure the fraction number changes of the cell cycle phase and to detect whether SB 202190 can induce apoptosis of C6 cells. RESULT The concentration of SB 202190 not more than 30 μmol·L -1 can promote C6 cells from G 1 phase to S phase but 60 μmol·L -1 induced apoptosis of C6 cells show Flow cytometry. The cells of apoptosis characterized by cellular shrinkage , membrane blebbing, nucleus condensation showed by the electron microscope and the intranucleosomal DNA frangment was detected by agrose gel electrophoresis. CONCLUSION SB 202190 can promote proliferation of C6 cells in lower concentration but induced apoptosis in higher concentration.