TNP-470对人结肠癌Lovo细胞增殖、细胞周期和凋亡的影响

Effects of TNP-470 on proliferation, the cell cycle and apoptosis in human colon cancer Lovo cells

目的 探讨TNP 4 70对体外培养的人结肠癌Lovo细胞增殖、细胞周期和凋亡的影响。方法 采用MTT法检测TNP 4 70对体外培养的Lovo细胞的生长抑制作用 ,流式细胞仪检测细胞周期分布及凋亡百分率。结果 TNP 4 70对Lovo细胞生长具有抑制作用 ,并存在量效和时间依赖性。流式细胞仪分析表明 :G0 /G1期细胞百分率上升 ,S期细胞百分率下降 ,增值指数 (PI)下降 ,凋亡率上升 ,电镜下可见典型的细胞凋亡形态改变。结论 TNP 4 70对Lovo细胞的增殖具有抑制作用 。

AIM To study the effect of TNP 470 on proliferation, cell cycle and apoptosis in the cultured human colon cancer Lovo cells. METHODS The growth inhibition of TNP 470 on Lovo cells was evaluated by an MTT.assay The effect of TNP 470 on cell cycle and apoptosis measured by flow cytometry, and ultrastructural feature of Lovo cells were observed with electromicroscope. RESULTS TNP 470 inhibited the growth of Lovo cells. flow cytometry analysis showed that G 0/G 1 phase rate was increased but S phase rate was decreased. Apoptosis rate of TNP 470 treated group was significant high than that of control and typical chang of apoptosis in Lovo cells was observed. CONCLUSION TNP 470 can inhibit proliferation of Lovo cells, and this inhibition is associated with cell cycle block and apoptosis.