HSP70高表达对K_(562)细胞化疗敏感性研究

Effects of HSP70 over expression on sensitivity of K_(562) cell to chemotherapy drugs

目的 研究化学因素引起的热应激蛋白 70 (HSP70 )升高对K56 2 细胞化疗药物体外敏感性的影响。方法用氯化镉诱导K56 2 细胞使其HSP70高表达 ,在高表达细胞中分别加入甲氨蝶呤 (MTX)、丝裂霉素C(MitomycinC)、顺氯氨铂 (PDD)、平阳霉素 (Bleomycin)等 4种化疗药物 ,药物浓度以临床一次用量的 1/ 2 5 0为中剂量 ,中剂量的 1/ 10、10倍分别为低剂量和高剂量 ,作用 16h后用MTT比色法检测细胞活力 ;HSP70用RT -PCR检测。结果 氯化镉可诱导细胞HSP70高表达 ,其中作用 4h后表达最高 ;HSP70高表达可以降低细胞对化疗药物的敏感性 ,HSP70表达水平越高 ,细胞对化疗药物的敏感性越低。结论 非热诱导的HSP70高表达可以降低肿瘤细胞对化疗药物的敏感性 。

Objective To study the effects of HSP70 over expression on the sensitivity of the K 562 cell to the chemotheraputic drugs.Methods The cadmium chloride was used to induce the K 562 cell HSP70 over expression,and then the chemotheraputic drugs which were methotrexate(MTX),mitomycin C,cisplatin(PDD) and bleomycin were added to the culture medium of the HSP70 over expression cells until 16 hours.Reversed transcription PCR(RT-PCR) was used to detect the HSP-70 mRNA and viability were measured by MTT methods.Results Cadimium chloride could induced the HSP70 over expression which showed high expression after induced for four hours.The higher HSP70 expression were,the lower the sensitivity of cells to chemotherapic drugs was.Conclusion The HSP70 induced by cadmium chloride could reduce the sensitivity of the cell to the chemotheraputic drugs and the effects were just as same as that of pre-heat.