麦胚黄酮类粗提取物抑制乳腺肿瘤作用机制

Study on inhibition of mammary tumor by flavonoids crude extract in wheat germ

目的 观察麦胚黄酮类粗提取物对DMBA诱导大鼠乳腺肿瘤的抑制作用并探讨其作用机理。方法 将 70只出生 5 0d的雌性SD大鼠随机分为 4组 ,分别为 :阴性对照组 (10只 ) ,乳腺癌模型组 (2 0只 ) ,低剂量组 (2 0只 )和高剂量组 (2 0只 )。将 15mg 7,12 -二甲基苯蒽 (DMBA)溶于 1 5ml植物油中 ,经灌胃分别给予乳腺癌模型组和实验组大鼠 ,阴性对照组给予等量植物油。 2周后重复 1次。低剂量组和高剂量组动物分别喂饲含 2 %和 10 %麦胚黄酮类粗提取物的饲料 ,阴性对照组和乳腺癌模型组饲以普通饲料。持续喂养 2 4周。 2 4周后断头处死大鼠 ,分别测定血液和肝脏中谷胱甘肽过氧化物酶 (GSH -Px)、超氧化物歧化酶 (SOD)、谷胱甘肽硫转移酶 (GST)活性及还原型谷胱甘肽 (GSH)、丙二醛 (MDA)含量。结果 实验组大鼠血液和肝脏中的GSH -Px、SOD、GST活性和GSH含量较乳腺癌模型组有明显增加 (P <0 0 5或P <0 0 1) ;MDA含量则明显下降 (P <0 0 5 )。实验组大鼠乳腺肿瘤的发生率低于乳腺癌模型组 ,但无显著性差异。结论 麦胚黄酮类粗提取物具有较强的抗氧化活性 。

Objective To investigate whether the flavonoids crude extrct in wheat germ could inhibite the mammary tumor induced by 7,12-dimethlben z〔a〕anthracene and clarify the possible inhibitory machenism.Methods Seventy female Sprague-Dawley(SD) rats aged 50 days were randomly divided into four groups:control group(10),mammary tumour group(20),low dose experimental group(20) and high dose experimental group(20).Except of rats in control group,each rat in other groups was given 15 mg 7,12-dimethylben z〔a〕 anthracene(DMBA) dissolved in 1.5ml plant oil for twice every other two week by gavage.While the rats in low and high dose experimental groups were given forage contained of 2% and 10% flavonoids crude extract of wheat germ respectively,rats in control and mammary tumor groups were given basic forage.After 24 weeks administered DMBA to rats,the glutathione peroxidase(GSH-Px),superoxide dismutase(SOD),glutathione S transferase(GST) activity and glutathion(GSH),malondialdehyde(MDA) level were measured respectively in blood and liver of rats.Results The flavonoids crude extract could enhance the GSH-Px,SOD,GST activity and GSH level in blood and liver of rats,decrease the MDA level (P<0 05).Conclusion The flavonoids crude extract had the anti-oxidation,which demonstrated one of mechanisms of chemophylactic effects on mammary tumor.