水通道与钠通道在小鼠胸腔液体转运中的作用

Effect of aquaporin-1 and sodium channel on pleural fluid transport in mice

目的 探讨水通道和钠通道在小鼠胸腔液体转运中的作用。方法 小鼠吸入麻醉后 ,胸膜腔内分别注入 0 2 5ml高渗和等渗液体 ,分别予以特布他林、阿米洛利、地塞米松和氯化汞 ,在不同时间点回收胸腔液体 ,测量其渗透压、容积和示踪剂12 5I 白蛋白计数。根据胸腔液体滤出率和吸收率变化 ,研究钠通道和水通道在胸腔液体转运中的作用。结果 阿米洛利抑制高渗液体引起的胸腔液体滤出和等渗液体吸收 (P <0 0 5 ) ;特布他林增加胸腔液体的滤出和吸收 (P <0 0 5 ) ;地塞米松增加高渗液体引起的胸腔液体的滤出 (P <0 0 5 ) ,氯化汞则起抑制作用 (P <0 0 5 ) ,地塞米松和氯化汞对胸腔液体的吸收无影响 (P >0 0 5 )。结论 水通道影响渗透压梯度引起的胸腔液体转运 ,对等渗液体的胸膜腔转运无明显影响。

Objective To investigate the effect of sodium channel and aquaporin 1 on pleural fluid transport*# Methods Mice were briefly anesthetized, and the pleural space was infused with 0 25 ml of hyperosmolar or isosmolar solution,with terbutaline or amiloride in instilled solution The mice were treated with either intraperitoneal dexamethasone or intravenous HgCl 2 Mice were euthanized at specified times for measurement of pleural fluid osmolality, volume and 125 I album in concentration The rate of pleural fluid filtration and clearance was calculated, which was used to indicate the effect of sodium channel regulator (amiloride and terbutaline) and aquaporin regulator ( dexamethasone and HgCl 2) on pleural fluid transport Results Amiloride decreased the osmotically driven water transport and isosmolar fluid clearance in pleural space ( P <0 05) Terbutaline increased osmotically driven water transport and isosmolar fluid clearance in pleural space ( P <0 05) HgCl 2 decreased , while dexamethasone increased , the osmotically driven water transport in pleural space ( P <0 05) HgCl 2 and dexamethason had no effect on pleural isosmolar fluid clearance ( P >0 05) Conclusions Sodium channel may play a role in osmotic and isosmolar fluid transport in pleural space Aqauporin 1 may play a role in osmotic fluid transport in pleural space, while having no effect on isosmolar fluid clearance