摘要
该文成功地将金溶胶、黄嘌呤氧化酶及Nafion依次修饰在微铂电极表面,研制成了一种新型的次黄嘌呤(Hx)微生物传感器。该微传感器响应快速、灵敏.对Hx具有良好的催化氧化性能。在 2.0×10^(-7)～2.0×10^(-5)mol/L浓度范围内,次黄嘌呤浓度与催化氧化电流成线性关系,检测限为1.0×10^(-7)mol/L(S/N=3)。该文还以该修饰微传感器为工作电极,研究了在L-精氨酸(L-Arg)和乙酰胆碱(Ach)的刺激下心肌细胞中次黄嘌呤的释放过程,得到令人满意的结果。
A novel hypoxanthine(Hx) microsensor was constructed. In this work, Nafion Xan-thine oxidase and Au colloid were immobilized on the surface of Pt microelectrode, respectively. The enzyme biosensor displayed a quick and sensitive response to Hx. In physiological condition, the biosensor showed a low detection limit, high selectivity and sensitivity for Hx determination. The oxidation current (measured by i-t)was linear with Hx concentration ranging from 2. 0× 10-7 to 2.0×10-5 mol/L with a calculated detection limit of 1.0×10-7 mol/L(S/N of 3). The biosensor should be promising for in vivo measurement of Hx without interferences and fouling. The change of Hx concentration in myocytes cardiac stimulated by L-Arg and Ach was also studied.
出处
《化学传感器》
CAS
2002年第4期8-17,共10页
Chemical Sensors
