RIA检测哮喘患者不同时期血清中细胞因子水平变化

The Changes of IL-8、IL-10、IL-12 and IgE in Serum of Patients With Asthma

采用放射免疫分析方法(RIA)和双抗夹心ELISA法检测了55例哮喘患者不同时期血清IL-8、IL-10、IL-12及IgE水平,以研究哮喘患者发作期与缓解期血清水平的变化及其在哮喘发病中的作用。结果显示:哮喘发作期患者血清IL-8、IgE水平显著高于缓解期及正常对照组(P<0.01),而IL-10、IL-12水平显著低于正常对照组(P<0.01)。这表明IL-8、IL-10及IL-12、IgE均参与了哮喘的发病,且IL-12降低与IgE升高有显著相关性。因此,IL-8、IL-10、IL-12及IgE可作为临床诊断哮喘、评价哮喘严重程度及疗效的指标。

To evaluate the relationship and the clinical significance between the serum IL8?IL10?IL12 and IgE in patients with asthma, the serum IL8, IL10 are measured by radioimmunolassay method and the serum IL12, IgE by ELISA in 55 patients with asthma. The level of serum IL8?IgE at stage of episode are significantly higher than that at stage of remission(P<0.01); the level of serum IL10,IL12 at stage of episode are signicantly lower than that at stage of remission(P<0.01). Linear regression shows that the decrease of IL12 relate to the increase of IgE. The results suggests that the change of the level of serum IL8?IL10?IL12 and IgE could be maker for the aggravation of asthma.