沙棘黄酮口服液中芦丁和5-羟色胺的毛细管区带电泳电化学检测

Determination of Rutin and Serotonin by Capillary Electrophoresiswith Electrochemical Detection

建立了毛细管区带电泳 -电化学检测法 (CE -ED)测定芦丁和5_羟色胺含量的方法 ,研究了电极电位、运行缓冲液的酸度和浓度、电泳电压及进样时间等因素对分离检测的影响,确定了最佳测定条件 ;以直径为300μm的碳圆盘电极为检测电极,电极电位为0.90V(vsSCE),在50mmol/L硼酸盐缓冲液(pH8.5)中,上述2组分在12min内完全分离 ,被分析物的电流响应与浓度在约3个数量级范围内呈良好线性关系,检出限分别为3×10-7 mol/L和8×10-8 mol/L ,7次测定含5.0×10-4 mol/L的芦丁和5_羟色胺的标准溶液,峰高的相对标准偏差分别为2.5 %和3.8 % ;

A method based on capillary electrophoresis with electrochemical detection(CE-ED) was developed for the determination of rutin and serotonin. The effects of some important factors such as the acidity and concentration of running buffer,the separation voltage,the injection time and detection potential were investigated to acquire optimum conditions. The working electrode was a 300 μm diameter carbon disc electrode positioned opposite the outlet of capillary. The analytes could be well separated within 10 min in a 60 cm length capillary at a separation voltage of 15 kV in a 50 mmol/L borate buffer(pH 8.5). The response was linear with concentration of the analytes over 3 orders of magnitude with detection limits(S/N=3) of 3×10-7 mol/L for rutin and 8×10-8 mol/L for serotonin. The proposed method demonstrated a good long _ term stability and repeatability with RSD of 2.5% and 3.8% for rutin and serotonin respectively in a repeatability study(n=7) at 5.0×10-4 mol/L level. It has been successfully applied for the determination of rutin and serotonin in Chinese traditional drug Oral Liquid of Flavones of Fructus Hippophae.