Nafion/Au溶胶自组装微铂传感器的研究及其在心肌细胞中NO水平测定中的应用

A Novel NO Microsensor and Its Application in the Detection of NO in Myocardial Cells

制备了一种新型的 Nafion/ Au溶胶修饰微铂传感器 ( Au溶胶颗粒的直径为 7～ 1 4 nm) ,并将该传感器应用于心肌细胞中 NO水平的研究 .实验结果表明 ,自组装制备的 Nafion/ Au溶胶修饰微传感器对 NO有较高的灵敏度和良好的选择性 ,在 1 .0× 1 0 - 7～ 4 .0× 1 0 - 5mol/ L浓度范围内 ,NO的催化氧化电流与其浓度呈良好线性关系 ,检测限为 5 .0× 1 0 - 8mol/ L.探讨了该修饰微传感器对 NO的催化机理 ,研究了在L-精氨酸和乙酰胆碱刺激下心肌细胞内的 NO释放 .

A novel sensitive, selective and stable nitric oxide(NO) microsensor is described, which is modified by nano Au colloid and Nafion. Determined by Atomic Forced Microscope, the diameter of Au colloid particles is from 7 to 14 nanometers. The detection of NO is based on the nano Au particles catalysis of NO oxidation at an anodic potential of +0 74 V(vs. SCE). The microsensor showed a low detection limit, high selectivity and sensitivity for NO determination. The oxidation current(measured by differential pulse amperometric technique) was linear with NO concentration ranging from 1 0×10 -7 to 4 0×10 -5 mol/L with a calculated detection limit of 5 0×10 -8 mol/L(S/N=3). Using the microsensor, the direct, real time production of NO in the myocardial cells was continuously measured, which showed the NO levels was increased by stimulating with L-arginine and acetylcholine.