巨桉芽器官离体培养与快繁体系建立的研究

RAPID PROPAGATION FROM SPROUT CULTURE IN VITRO OF EUCALYPTUS GRANDIS

研究了以巨桉 (Eucalyptusgrandis)优树的带芽茎段为外植体诱导丛生芽发生以及植株再生的过程 ,对培养过程中新芽及丛生芽的发生类型进行了探讨。通过正交实验及单因素试验 ,确定了巨桉快繁体系的最适培养条件 :(1 )初代培养基 :S +BA0 5mg·L- 1 +NAA0 0 5mg·L- 1 +蔗糖 3 % ;(2 )丛生苗诱导培养基 :S +BA1 0mg·L- 1 +NAA0 0 5mg·L- 1 +蔗糖 3 % ;(3 )有效苗诱导培养基 :S+BA0 5mg·L- 1 +NAA0 1mg·L- 1 +生物素 2 0 +蔗糖 4% ;(4 )壮苗培养基 :MS(铁盐、有机物 1 5倍 ) +蔗糖 4% ;(5 )生根培养基 :1 2MS +ABT生根粉 (1号 ) 1 0mg·L- 1 +蔗糖 2 %。

This paper mainly dealt with the study on shoot organogenesis culture in vitro and plantlet regeneration from sprout explants of Eucalyptus grandis. The organogenesis types of regeneration shoots and clump shoots were discussed. The optimal culture conditions were established by orthogonal tests and single factor tests as follows: (1)Initial media: S+0 5 mg·L -1 BA+0 05 mg·L -1 NAA+3%sucrose;(2)Clump bud induction media: S+1 0 mg·L -1 BA+0 05 mg·L -1 NAA+3%sucrose;(3)Efficiency seedling induction media: S+0 5 mg·L -1 BA+0 1 mg·L -1 NAA+2 0 mg·L -1 biotin+3%sucrose;(4)Robust seedling media: MS(1 5 strength of Fe and organic compound)+4%sucrose;(5)Rooting media:1/2MS+1 0 mg·L -1 ABT+2%sucrose.