抗传染性支气管炎病毒中药的研究

Study on the selection and prevention cure effects of the Chinese medicinal herbs against IBV

用细胞培养法、鸡胚培养法对车前子、麦冬等 4 0种中药进行抗传染性支气管炎病毒(IBV)的筛选试验。通过细胞病变 (CPE)抑制试验发现 ,石榴皮等 5种中药有抑制CPE的作用。培养细胞有 2 0 %发生CPE ,认为这些中药有抗IBV的作用。应用空斑形成抑制试验对 5种抗IBV中药进行复筛 ,结果表明 5种中药均有不同程度的抑制病毒空斑形成作用 ,其中以石榴皮等 2种中药作用较强 ,空斑形成抑制率可达 98%以上。应用鸡胚培养法对 5种中药进行抗IBV试验 ,结果发现 5种中药均显著地减少了鸡胚病变及胚体的死亡 ,其中以石榴皮等 2种中药抑制胚体病变的作用最强。通过不同加药时间观察到在接毒前 4 8h给药 ,有 2种中药具有明显抑制CPE的作用 ;接毒同时加药以及接毒后 1h加药 ,这 5种中药均能明显抑制CPE。

Experiment against IBV was carried out by cell culture on 40 Chinese medicinal herbs.Initial selection from 40 Chinese medicine herbsby CPE inhibition assay showed that only 5 Chinese medicinal herbs had the obvious effect on IBV. Further selection from the five Chinese medicinal herbs by plaque forming experiment showed that they all could inhibit the forming of the virus plaque in which Shi liupi had more obvious effects. Experiment against IBV by chicken embryo culture showed that the five kinds of herbs all decreased the pathological changes in which Shi liupi had most intensity. Different treating time had different effects on CPE inhibition, such as when treating 48 hours before planting IBV in cell monolayer there were 2 herbs which had significant inhibition effect.