山葡萄种质RAPD研究

RAPD Analysis of Intraspecific Polymorphism in Vitis amurensis

采用RAPD技术对山葡萄 7份种质进行分类鉴定研究。用 12个引物共扩增出 74个位点 ,平均每条引物产生 6个位点 ,其中 4 8个位点为多态性位点 ,多态性百分率为 6 5 %。不同引物扩增的位点数不同 ,范围在 2～ 9个之间。对 7个供试材料间遗传相似系数采用UPGMA法聚类 ,结果表明 :如取阈值T =0 .4 ,供试材料可分为 4组 ,第一组只有雄性山葡萄种质 1- 2 ;第二组包括雌能花山葡萄左山一和 730 93;第三组为两性花山葡萄双丰 ;第四组包括 3个雌能花山葡萄种质 75 0 2 3、左山二、730 6 4。

random primers were screening out from 7 germplasm of V. amurensis samples whose flower styles were not the same. Among these primers, 12 primers whose bands style was clean and stable were selected for applying . The genomic DNA fingerprinting was all acquired in 7 samples by using 12 primers, and the polymorphism was different for different primers. The bands that the different primer amplified was different and the band number ranged from 2 to 9.11 of 12 primers in 7 samples could produce 1～5 same bands. 26 of 74 bands were monomorphic bands. The phenomenon suggested the similarity and variability of genetic background of V. amurensis . The genetic similarity coefficient of 7 samples was classified by UPGMA method. The result was similar to morphology taxonomy, which suggested that RAPD was effective in identifying gene type.