鼻息肉中干细胞因子mRNA的检测及其意义

Expression of stem cell factor mRNA and the role in nasal polyps

目的:检测鼻息肉组织和下鼻甲黏膜组织中的干细胞因子(stemcellfactor,SCF)mRNA水平的表达,探讨其在慢性炎症过程中的作用。方法:取10例鼻息肉切除术的鼻息肉标本和7例行下鼻甲切除术的下鼻甲黏膜,用半定量的逆转录-聚合酶链反应(reversetranscription-polymerasechainreaction,RT-PCR)方法检测SCFmRNA的表达。结果RT-PCR结果显示10例鼻息肉中SCF的表达0.864±0.165较7例下鼻甲组织0.152±0.217明显升高,t检验P<0.01,差异有显著性意义。结论:鼻息肉组织中SCF的表达显著升高,推测SCF通过参与活化肥大细胞和嗜酸性粒细胞的过程,在鼻息肉的形成中具有重要的作用。

Objective To explore the pathogenesis of nasal polyps.The aim of this study is to detect the expression of stem cell factor (SCF)in nasal polyp tissue,and to compare these findings with that in normal nasal turbinate.Method We examined the expression of SCF in human nasal polyps from patients undergoing nasal polypectomy (n=10)and nasal turbinectomy (6=7).The SCF mRNA expression was detemined by semi-quantitative reverse transcription-polymerase chain reaction(RT-PCR).Re -sults The SCF mRNA expression was significantly stronger in the nasal polyps(NP)than that in turbinate tissues(P<0.01).Con-clusions SCF expression is upregulated in NP,which indicate that SCF may play a potential role in the formation and growth of NP.