体外培养的高血压病动脉平滑肌细胞端粒酶活性检测及其在发病学上的意义

The Significance of Telomerase Activity Detection in Cutured Arteriolar Smooth Muscle Cells to Pathogenesis of Essentional Hypertension

目的:探讨体外培养的高血压病动脉平滑肌细胞端粒酶活性与高血压的发病关系。方法:采用PCR-ELISA法检测10例高血压病患者和8例血压正常者体外培养的肠系膜动脉平滑肌细胞(SMC)端粒酶活性。结果:10例高血压端粒酶阳性表达9例,阳性率90％。对照组血压正常病人8例,端粒酶阳性表达2例,阳性率25％。差异有显著性(P<0.05)。结论:体外培养的高血压病动脉平滑肌细胞有端粒酶活性的表达,端粒酶活性可能与高血压发病有关。

Objective: To study the relationship between telomerase activity in cultured arteriolar smooth muscle cell of essential hypertension patients and pathogenesis of essential hypertension. Methods:PCR- ELISA was applied to detect telomerase activity of cultured mesenteric arteriolar smooth muscle cells in 10 cases of essential hypertension and 8 cases of normal blood pressure. Results: Positive rate of telomerase activity was 90% (9/10) in 10 cases of hypertension and 25% (2/8) in cases of normal blood pressure. Conclusion: Telomerase activity expression can be observed in cultured arteriolar smooth muscle cells in essential hypertension patients . Telomerase activity may be involved in the pathogenesis of essential hypertension.