电针对胶原性关节炎大鼠的镇痛效应和抗体、IL—1β水平的影响

Influences of Electroacupuncture on Pain Threshold and Antiˉcollagen Antibody,ILˉ1βin CIA Rats

目的 :观察电针对胶原诱导性关节炎大鼠痛阈、β 内啡肽、抗II型胶原抗体及白介素 1β 水平(IL 1β)等的影响 ,以探索电针镇痛与免疫调节的双重作用及其相关性。方法 :Wistar雄性大鼠经牛II型胶原皮内注射接种诱导胶原性关节炎动物模型 ,在造模后第3天起在“足三里”穴进行电针刺激 ,连续19天。采用辐射热甩尾法测定大鼠痛阈 ,Wood氏法评估关节炎指数 ,酶联免疫吸附实验(ELISA)检测关节炎大鼠血清抗II型胶原抗体水平和脾脏白介素 1β 含量 ,放射免疫分析(RIA)法检测大鼠脑组织与脾脏中 β 内啡肽水平。结果 :电针能明显提高胶原诱导性关节炎大鼠的痛阈 ,对不同时期胶原免疫大鼠的痛阈有显著的即时升高效应 ,并能相应地增加大鼠脑组织和脾脏中 β 内啡肽的含量。电针不仅能有效地减少造模大鼠的肢体累及率 ,更明显地降低大鼠的关节炎指数和血清中抗II型胶原抗体水平。同时电针能显著抑制胶原诱导的大鼠脾组织中IL 1β 的水平。结论 :本研究说明 ,电针在对疼痛和免疫失调相兼疾病的治疗中不仅能镇痛 ,同时进行相应的免疫调节 。

Objective:The influences of electroacupuncture(EA)on the pain threshold,NK/LAK cell activities in tumorˉborne rats and painshold,βˉendophine,antiˉC II antibody and interleukinˉ1β(ILˉ1β)levels in CIA rats were observed to investigate the double effects of EA on analgesia and immune regulation as well as their correlations.Methods:Male Wistar rats injected intradermally by bovine type II collagen were induced to collagen arthritis.EA treatment started3days after immunization and applied at Zusanli(ST36)was given for19days We used TFTto measure the pain threshold,Wood's method to evaluate the arthritic index,ELISA to measure the serum antiˉC II antibody and splenic ILˉ1βlevels,and RIA the brain and splenicβˉendophine levels.Results:EA obviously raised the pain threshold of CIA rats,and achieved the immediate analgesic effect in the treatment of different periods.Theβˉendophine levels in brain tissue and spleen of collagen immunized rats were accordingly liftˉed.EA stimulation not only significantly reduced the affected limbs of arthritis,but also lowered the arthritic index and serum antiˉC II antibody level.The concentration of splenic ILˉ1βinduced by collagen injection was notably inhibited.Conclusion:EA possessed strong analgesic effect on experimental rheumatoid arthritis,and activated the release of endogenous opiates.Moreover,EA controlled the disease condition of arthritic rats,remarkably reduced antiˉcollagen antibody and inflammatory cytokine levels.The present study indicates that in the treatment of disorders with pain and immune disturbance,EA therapy can get both effects of analgesia and immune regulation.These two effects are related each other in the treatment with EA.