人类survivin基因的克隆及在胃粘膜中表达的初步分析

Expression of human anti-apoptotic gene survivin and its splice in normal human gastric tissue and gastric cancer

目的 克隆人类 Survivin(SVV)基因并分析其在胃粘膜中的表达情况。方法 采用逆转录 -聚合酶链反应从人胃癌组织中克隆 SVV基因 ,地高辛标记 c RNA探针 ,原位杂交检测其在胃粘膜中的表达。结果 得到两个 SVV基因 c DNA克隆、即 SVV- S4 A与 SVV- S1B,前者与已知 SVV基因 c DNA序列相同 ,后者发生了 SVV第 3外显子丢失。原位杂交显示 SVV- S4 A主要表达于胃癌细胞的胞质 ,SVV- S1B主要表达于正常胃组织。结论 SVV- S4 A和 SVV-

Objective: To analyze the expression of human anti-apoptotic gene survivin (SVV) in normal human gastric tissues and gastric cancer. Methods: SVV cDNA clone was obtained from human gastric cancer tissues by virtue of RT-PCR, using Dig-marked cRNA probe in situ hybridization to analyze its expression in normal human gastric tissues and gastric cancer. Results: Two SVV cDNA clones, SVV-S4A and SVV-S1B were obtained. The sequence of the former is identical to that of the well-known SVV cDNA; however, in the sequence of the latter, the third exon was missed, i.e., there are only two exons in SVV-S1B. In situ hybridization showed that SVV-S4A is mainly expressed in gastric cancer tissues whereas SVV-S1B is mostly expressed in normal gastric tissues. Conclusion: There is difference between SVV-S4A and SVV-S1B in respect to their characteristics of expression in gastric cancer and normal gastric tissues.