云南藏族四个短串联重复序列基因座的基因频率

Gene frequencies of 4 STR loci in Tibetan population of Yunnan province

目的 了解云南藏族 D2 1S11、D8S1179、D16 S5 39和 L PL 4个短串联重复序列基因座的基因频率。方法 采用 4个 STR基因座引物在同一反应体系中互不干扰的复合扩增、变性聚丙烯酰胺凝胶电泳及银染法显色技术 ,对 10 5名云南藏族个体 4个 STR基因座进行了基因频率调查。结果 观察到D2 1S11基因座的 13个等位基因和 33个基因型 ;D8S1179基因座的 8个等位基因和 2 1个基因型 ;D16 S5 39基因座的 7个等位基因和 16个基因型 ;L PL 基因座的 6个等位基因和 9个基因型。结论 4个基因座的基因型分布均符合 Hardy- Weinberg平衡定律 ,且在藏族群体中有较好的多态性分布 ,适用于法医个体识别和亲权鉴定。

Objective: To investigate the gene frequencies of 4 STR loci in Tibetan population of Yunnan. Methods: Multiple polymerase chain reaction (PCR), denaturing polyacrylamide gel electrophoresis and silver staining were used to detect D21S11, D8S1179, D16S539 and LPL loci. DNA samples collected from 105 unrelated Tibetan individuals in Yunnan province were analyzed. Results: At D21S11, D8S1179, D16S539 and LPL loci, 13, 8, 7, 6 alleles and 33, 21, 16 and 9 genotypes were observed, respectively. The genotype distribution of the 4 STR was in accordance with the Hardy-Weinberg equilibrium. Conclusion: The high combined discrimination power and exclusion power of the four loci in Tibetan population make multi-PCR detection a valuable tool for forensic identity, genetics and anthropology.