摘要
目的 探讨牙本质磷蛋白 (dentinphosphoprotein ,DPP)诱导矿化的作用。方法 在体外矿化系统中利用人DPP与EAH Sepharose 4B凝胶颗粒结合进行诱导矿化实验 ,通过扫描电镜观察生成物的形貌变化 ,X线衍射及等离子发射光谱分析生成物的组分和结构。结果 结合有人DPP的凝胶颗粒表面有矿化物形成 ,该矿化物钙磷比约为 1 33 ,其X线衍射结果与羟基磷灰石更为相似。
Objective To investigate the effect of dentin phosphoprotein(DPP) in inducing dentinal mineralization Methods Human DPP was combined with EAH Sepharose 4B beads and its function of inducing mineralization was studied in mineralization system in vitro The mineral formed on the surface of the beads was analyzed by scanning electron microscopy (SEM) and the structure was analyzed by X ray diffraction and plasma emission spectrum Results There was mineral formed on the beads with combined DPP and the mineral was calcium phosphates whose ratio of calcium to phosphate was 1 33 The diffractogram of the formed mineral was more similar to hydroxyapatite than to other calcium phosphates Conclusion When tightly combined with certain support substance, human DPP can induce mineralization
出处
《中华口腔医学杂志》
CAS
CSCD
北大核心
2003年第1期56-58,I004,共4页
Chinese Journal of Stomatology
基金
北京市自然科学基金资助项目 ( 9752 0 0 8)
