超高分子量聚DL乳酸对成骨细胞活性及新骨生成的影响

Influence of super high molecular weight poly D,L-lactic acid on viability and new bone formation of osteoblasts

目的 研究超高分子量聚DL乳酸材料对成骨细胞活性及其在骨创愈合过程中对新骨生成的影响。方法 ①体外实验 :在超高分子量聚DL乳酸材料浸提液和标准培养基中培养乳鼠成骨细胞 ,采用四甲基偶氮唑盐微量酶反应比色法检测成骨细胞的活性和功能。②体内实验 :在犬下颌骨体部制造人工骨折模型 ,用聚DL乳酸制作的夹板螺钉固定骨折处 ,在扫描电子显微镜下观察骨折线处的新骨沉积情况。结果 ①超高分子量聚DL乳酸对成骨细胞在 3d时毒性为 3 % ,在 1周、2周时均为 0 ;实验组和对照组的A值在 3d、1周、3周时差异均无显著性。②术后 3个月时 ,电镜下可见聚DL乳酸材料与骨组织之间有大量新骨形成。结论 超高分子量聚DL乳酸对成骨细胞的活性及新骨生成均无影响 。

Objective To investigate the influence of the viability and new bone formation of osteoblasts by the super high molecular weight poly D,L lactic acid (SHMW PDLLA) Methods 1 The osteoblasts derived from neonatal rat were grown and maintained at steep of SHMW PDLLA and normal culture medium The viability and function of the osteoblasts were measured with MTT array 2 The plate and screws made of SHMW PDLLA were implanted and fixed at the artificial fractured mandible of dogs Specimens were gained at 3 and 6 months and examined with macroscopy and SEM Results 1 There is no significant difference of OD values between the experimental group and the control group ( P >0 05) The SHMW PDLLA isn′t toxic to osteoblast at 1 week and 2 weeks, and the toxicity is 3% at 3 days 2 There were a lot of new bone formed between the implanted SHMW PDLLA plate and bone tissues under SEM Conclusion SHMW PDLLA hasn′t pathological influence on the viability and new bone formation of osteoblasts and it is feasible in tissue engineering of bone