哮喘患者白细胞介素(IL)-12和IL-13的变化及糖皮质激素对其的影响

Changes of serum interleukin (IL)-12 and IL-13 in asthmatic patients and regulatory effects of glucocorticoids on them

目的 观察哮喘患者血清中白细胞介素 (IL) 12和IL 13水平的变化及糖皮质激素对其的影响。方法 采用ELISA法分别检测中度哮喘急性发作期患者 (2 5例 )口服泼尼松治疗 1周前后、缓解期患者 (2 0例 )和健康对照组者 (15例 )血清中IL 12和IL 13水平 ,并同时测 1秒钟用力呼气容积(FEV1)占预计值的百分比和气道阻力 (R5)占预计值的百分比。结果 IL 12水平急性发作期治疗前[(5 8 5± 14 2 )ng/L]较缓解期 [(71 3± 16 2 )ng/L]为低 (P <0 0 5 ) ,与健康对照组 [(85 5± 13 1)ng/L]、急性发作期治疗后 [(79 3± 19 1)ng/L]比较 ,差异均有显著性 (P <0 0 1)。IL 13水平急性发作期治疗前 [(131 3± 2 8 4 )ng/L]较缓解期 [(113 1± 2 6 5 )ng/L]为高 (P <0 0 5 ) ,与健康对照组 [(92 3± 14 4 )ng/L]、急性发作期治疗后 [(84 1± 19 8)ng/L]比较 ,差异均有显著性 (P <0 0 1)。FEV1占预计值的百分比下降和R5占预计值的百分比升高 (P <0 0 1)。直线相关分析表明 ,血清中IL 12与FEV1占预计值的百分比呈正相关 (r=0 4 85 ,P <0 0 5 ) ,与R5占预计值的百分比呈负相关 (r =- 0 5 16 ,P<0 0 5 ) ,与IL 13呈负相关 (r =- 0 5 4 9,P <0 0 1) ;IL 13与FEV1占预计值的百分比呈负相关 (r =- 0 4 93,P

Objective To observe the changes of serum interleukin(IL) 12, IL 13 and lung function in asthmatic patients and to evaluate the influence of glucocorticoid on them Methods The serum samples were obtained from(1)25 asthmatic patients with acute asthma attack before and after one week course of oral prednisone,(2)20 asthmatic patients in remission stage, and (3)15 healthy volunteers Serum IL 12 and IL 13 were determined with sandwich ELISA Lung ventilatory function forced expiratory volume in one second(FEV 1 ) and respiratory impedance airway resistance(R 5) were measured in all patients Results (1)Serum level of IL 12 in asthma attack group was significantly lower than that in asthma remission group( P <0 05); both were significantly lower than that in normal control group( P <0 01); in asthma attack group IL 12 level after prednisone treatment was significantly higher than that before treatment( P <0 01 ) (2) Serum level of IL 13 in asthma attack group was significantly higher than that in asthma remission group( P <0 01 ); both were significantly higher than that in normal control group( P <0 01 ); in asthma attack group IL 13 level after prednisone treatment was significantly lower than that before treatment( P <0 01 ) Correlation analysis showed that the serum level of IL 12 was positively correlated to FEV 1 and negatively correlated to R 5 and to serum level of IL 13( r 1=0 458, r 2=-0 516, and r 3=-0 549, respectively; P <0 05 and P <0 01 ); the serum level of IL 13 was negatively correlated to FEV 1 and positively correlated to R 5 ( r 1=-0 493,and r 2=0 528,respectively; P <0 05 ) Conclusion The secretion of IL 12 and IL 13 was impaired in asthma with a significant increase in serum level of IL 13 and decrease in serum level of IL 12 Glucocorticoid could downregulate the serum level of IL 13 and upregulate the serum level of IL 12, redress the imbalance of IL 12/IL 13, and improve lung fumction in asthmatic patients