胃液固有荧光光谱对胃癌诊断价值的初步研究

The value of autofluorescence spectrum analysis of gastric juice in diagnosing gastric carcinoma:a preliminary result

目的 显示胃癌患者及非癌患者胃液固有荧光光谱的荧光强度的差异 ,以期用于胃癌的筛查及诊断。方法 收集 2 0 2例各种胃内疾病患者的胃液 ,经 1∶10稀释后 ,检测激发波长为 2 88nm ,发射波长范围为 30 0～ 80 0nm的固有荧光光谱。用CARTV2 0统计软件建立判别模型 ,计算先验概率与后验概率。结果 各种胃内疾病患者的胃液固有荧光光谱均有 3个峰 (发射波长分别为32 0～ 36 0nm、5 76nm及 6 70～ 6 90nm) ,胃癌患者的第一荧光峰 (发射波长为 32 0～ 36 0nm)的强度 (P1FI)较其他胃内良性疾病患者明显增强。以P1FI≥ 111 80作为判别指标 ,用于胃癌诊断的先验概率的敏感度为 91 4 % ,特异度为 83 2 % ,准确度为 84 7%。其后验概率的敏感度为 85 7% ,特异度为82 6 % ,准确度为 83 1%。

Objective To reveal the difference of autofluorescence spectrums of gastric juice derived from malignancy and benignancy for screening and diagnosis of gastric carcinoma Method Gastric juice from 202 patients with different gastric diseases were collected, then detected their autofluorescence spectrums (the excitation wavelength is 288 nm, whereas the range of emission wavelength is 300-800nm)after diluted by 1∶10 The diagnostic model for gastric cancer was made by Classification and Regression Trees V2 0 software Results There were three peaks (the emission wavelength were 320-360 nm,576 nm and 670-690 nm respectively) in the autofluorescence spectrums of all patients, though the intensity of the first peak (with the emission wavelength of 320 360 nm) were enhanced distinctively in malignance than those in benignancy The diagnostic model's sensitivity and specificity of prior probability were 91 4% and 83 2% respectively, whereas the sensitivity and specificity of posterior probability were 85 7% and 82 6% Conclusion Detection of autofluorescence spectrum of gastric juice has great prospect in the diagnosis and screening gastric carcinoma