胃癌组织NOS和VEGF表达与癌细胞增殖相关性的研究

Correlation of Cancer cell Proliferation and Expression of Vascular Endothelial Growth Factor and Nitric Oxide Synthase in Human Gastric Cancer

目的:研究人胃癌组织血管内皮生长因子(VEGF)、诱导型一氧化氮合酶(iNOS)、内皮型一氧化氮合酶(eNOS)的表达与癌细胞增殖的相关性。方法:应用免疫组化方法检测34例胃癌手术切除标本VEGF、iNOS、eNOS和增殖细胞核抗原(PCNA)的分布及表达。结果:①31例胃癌组织表达VEGF,25例(73.5%)表达iNOS,28例(82.4%)表达eNOS;②VEGF与iNOS的表达具有明显相关性,VEGF与eNOS的表达无明显相关性;③表达VEGF的胃癌PCNA标记指数(PLI)明显高于不表达VEGF的胃癌,表达iNOS的胃癌PLI明显高于不表达iNOS的胃癌,表达eNOS的胃癌PLI与不表达eNOS的胃癌无显著性差异(P<0.05)。结论:①VEGF与iNOS的表达具有明显相关性,说明iNOS在VEGF的生成和发挥作用过程中起重要作用;②PLI随着VEGF和iNOS表达的增加而增加,说明二者对胃癌细胞增殖具有促进作用。

Objective:To study the relation of protein expression of inducible nitric oxide synthase(iNOS),endothelial nitric oxide synthase(eNOS)and vascular endothelial growth factor(VEGF)as related to gastric cancer(GC)proliferation.Methods:GC tissue specimens were obtained from34patients undergoing operation,by using the immunohistochemistry technique,the expression of iNOS,eNOS and VEGF and the expression and distribution of PCNA(proliferating cell nuclear antigen,PCNA)were studied.Results:①Positive iNOS,eNOS and VEGF immunostaining were detected in25(73.5%),28(82.4%)and31(91.2%)patients with GC respectively.②Expression of VEGF bore a significant positive relation to iNOS,but not eNOS.③PLI(PCNA labelling index in VEGF and iNOS positive cases was significantly higher than that in VEGF and iNOS negative cases.No difference PLI level was noted in both eNOS positive and negative cases.Conclusions:①VEGF and iNOS bear a significant positive correlationship in human GC,showing that iNOS,rather than eNOS,plays an important role in VEGF production.②PLI increased with the increment of VEGF and iNOS expression.VEGF and iNOS are known to enhance gastric cancer cell proliferation.