耐药相关基因拓扑异构酶-Ⅱα在人胰腺癌细胞株中的表达

Expression of Drug Resistant Associated Gene TOPO-Ⅱαin Human Pancreatic Cancer Cell Lines.

目的:探讨耐药相关基因拓扑异构酶鄄Ⅱα((TOPO鄄Ⅱα)在人胰腺癌细胞株中的表达。方法:通过逆转录鄄聚合酶链反应(RT鄄PCR)和Western鄄blot方法测定6株人胰腺癌细胞株(SW1990、Capan鄄2、AsPC鄄1、MiAPaCa鄄2、PANC鄄1、P3)中的TOPO鄄Ⅱα在基因和蛋白水平上的表达。结果:①TOPO鄄Ⅱα基因的表达以SW1990最高,PANC鄄1最低,与其他细胞株相比均具有显著性差异(P<0.05);②TOPO鄄Ⅱα蛋白的表达以SW1990、Capan鄄2较高,AsPC鄄1较低。SW1990,Capan鄄2与其余4株之间存在着显著性差异(P<0.05),而SW1990与Capan鄄2之间无显著性差异(P>0.05);AsPC鄄1、MiAPaCa鄄2、PANC鄄1、P3之间均无显著性差异(P>0.05)。结论:胰腺癌细胞对TOPO鄄Ⅱα抑制剂的低敏感性与TOPO鄄Ⅱα蛋白的低水平表达有关,TOPO鄄Ⅱα可能参与了胰腺癌对化疗的耐药。

Objective:To investigate the expression of TOPOⅡαin six Human pancreatic cancer cell lines(SW1990,Capan2,AsPC1,MiAPaCa2,PANC1,P3)as well as the relationship between TOPOⅡαand drug resistance in pancreatic cancer.Methods:The expression of TOPOⅡαon genetic level was determined by semiquantitative RTPCR.The expression of TOPOⅡαon protein level was determined by Western blot.Results:SW1990had the highest level of TOPOⅡαgene expression,PANC1had the lowest level of expression;there was significant difference between SW1990and PANC1compared to the other four cell lines.Capan2and SW1990had the relative higher levels of TOPOⅡαprotein expression,and AsPC1had the lowest.There was significant difference between Capan2,SW1990and the other four cell lines(P<0.05);there was no significant difference between Capan2and SW1990(P>0.05),there was also no significant difference among the other four cell lines(P>0.05).Conclusions:All showed that six cell lines with different expression levels in gene and protein of TOPOⅡα.Considering results of our previous studies,the low sensitivity of pancreatic cancer cells to inhibitors of TOPOⅡαwas related with low expression of TOPOⅡαand has a relationship with multidrug resistance in pancreatic cancer.