摘要
目的 :建立丹参及其亲缘植物中丹参酮类与丹酚酸类成分同时检测的含量测定方法 ,为丹参的质量控制及临床替代用药提供科学依据。方法 :采用高效液相色谱法测定 9种主要成分的含量。色谱柱为ShimadzuCLC ODS柱 (15 0mm× 6mm ,5 μm) ,流动相为乙腈、0 5 %甲酸水溶液梯度洗脱 ,流速为 0 8mL·min-1,检测波长 0～ 2 5min为 2 81nm ,2 5min后为 2 5 4nm。结果 :9种成分在线性范围内均呈现良好的线性 (r>0 999)。结论 :丹参及其亲缘植物丹参酮类、丹酚酸类的含量差异显著 ,同种不同产地的样品之间也存在明显差异 ,丹参的质量控制是一个亟待解决的问题 ;云南鼠尾、红根草中几乎未检出丹参酮类成分 。
A method of gradient-elution HPLC with UV detection was developed for theanalysis of nine major constituents of Salvia species, a commonly used TCM herb, namely danshensu,protocatechuic acid, protocatechualdehyde, salviano-lic acid B, methyltanshinone, dihydrotanshinone,cryptotanshinone, tanshinoneⅠand tanshinoneⅡ_A. In the present study, a Shimadzu CLC-ODS column(150 mm x 6 mm, 5 μm) was utilized and 0.5% formic acid (A) and acetonitrile (B) were used forgradient elution at a total flow rate of 0.8 mL· min^(-1). All calibration curves showed goodlinear regression ( r > 0.999) within test ranges. Extraction was conducted by refluxing methanol(10 mL) with dried herb (0.5 g) for 1.0 h.The assay was simple, convenient and reproducible. Theproposed method was successfully applied to the determination of nine major constituents in thirteenSalvia. species and the results showed that the contents of Salvia components vary in differentspecies and origin. Tanshinone was hardly detected in S. yunnanensis and S. prionitis, thereforethey are not suitable for clinical use as Danshen.
