Quantitative Study of Basic Fibroblast Growth Factor in Vitreous with Proliferative Viteoretinopathy

To quantitatively study basic fibroblast growth factor(bFGF) in the vitreous of proliferative vitreoretinopathy(PVR) in order to understand the role of bFGF in the development of PVR.Method:High sensitive sandwich enzyme immunoassay technique(ELISA) was used to measure bFGF level in vitreous of normal eyes,the eyes of PVR-C or PVR-D grade,eyes of vitreous hemorrhage and the serum lkevels of bFGF in PVR-D patients.Results:The levels of bFGF in the vitreous were:median 5.20ng/L,quartile 15.47ng/L in 20 normal eyes;median 3.12ng/L,quartile 10.48ng/L in 35 PVR-C eyes;median 46.56ng/L,quartile 113.96ng/L in 26 PVR-D eyes;median 1.40ng/L,quartile 6.25ng/L in 25 vitreous hemorrhage eyes.The vitreous bFGF level in PVR-D group was significantly highter than that in the normal group ,PVR-C group and vitreous hemorrhage group(p<0.01).The mean of serum-bFGF level was 18.33±3.39ng/L.The vitroues bFGF level of PVR-D group was significantly higher than serum-bFGF level (P<0.01).And the vitreous-bFGF level in PVR-D group was significantly hgher in larger retinal tear subgroup.Concvlusion:The results suggested that bFGF is involved in the development of PVR.Eye Science 2000;16:7-10.