摘要
Objective: To investigate whether hPTH1-34 regulate the synthesis of fibronectin (FN) from cultured rat mesangial cells and its possible mechanism. Methods: (1) MCs seeded at a density of 1 × 104 per well in 24-well plates were treated with medium containing various concentrations of hPTH1-34(10-12 mol/l -10-8 mol/l) for 6 h, 12 h, 24 h and 48 h, control cells were treated with vehicle only. The FN levels (in the supernatant) were measured by ELISA assay. (2) MCs were co-cultured with 10 ng/l of anti-TGF-β antibody and various concentrations of hPTH1-34(10-12 mol/l - 10-8 mol/l ). Forty-eight hours later, FN were tested by ELISA. (4) MCs were co-cultured with 10 ng/l of anti-TGF-β antibody and 10-8 mol/l hPTH1-34 for 6 h, 12 h, 24 h and 48 h and then FN were tested. Results: (1) hPTH1-34 stimulated FN synthesis in a dose-and time-dependent way with a peak at 10-8 mol/l (P<0. 01). (2) Anti-TGF-β antibody inhibited the stimulation effect of hPTH1-34, on synthesis of FN in cultured rat mesangial cells (P<0. 05). Conclusion: hPTH1-34 up-regulates FN synthesis in cultured rat mesangial cells via TGF-β, suggesting that PTH may play an important role in deteriorating the residual renal function at the early stage of chronic renal disease.
Abstract Objective:To investigate whether hPTH1-34 regulate the synthesis of fibronectin(FN) from cul-trued rat mesangial cells and its possible mechanism.Methods:(1) MCs seeded at a density of 1×10^4 per well in 24-well plates were treated with medium containing various concentrations of hPTH1-34(10^-12mol/l-10^-8mol/l)for 6h,12h,24h and 48h,control cells were treated with vehicle only.The FN levels (in the supernatant)were measured by ELISA assay.(2) MCs were co-cultured with 10ng/l of anti-TGF-βanti-body and various concentrations of hPTH1-34(10^-12mol/l-10^-6mol/l).Forty-eight hours later, FN were tested by ELISA.(4)MCs were co-cultured with 10ng/l of anti-TGF-β antibody and 10^-8 mol/l hPTH1-34 for 6h,12h,24h and 48h and then FN were tested.Results(1)hPTH1-34 stimulated FN synthesis in a dose-and time-dependent way with a peak at 10^-8 mol/l(P<0.01).(2)Anti-TGF-β antibody inhibited the stimu-lation effect of hPTH1-34 on synthesis of FN in cultured rat mesangial cells(P<0.05).Conclusion:hPTH1-34 up-regulates FN synthesis in cultured rat mesangial cells via TGF-β ,suggesting that PTH may play an im-portant role in deteriorating the residual renal function at the early stage of chroic renal disease.
