维拉帕米和超氧化物歧化酶对体外冲击波肾损伤的保护作用

The protective effects of verapamil and SOD on ESW-induced kidney damage

目的 探讨钙离子拮抗剂维拉帕米和自由基清除剂对体外冲击波 (ESW )肾损伤的保护作用。 方法 12 0只SD大鼠单肾模型随机分为 6组 ,在单用维拉帕米和 (或 )联用不同剂量超氧化物歧化酶 (SOD)后以JDPN Ⅴ型碎石机 14kV对鼠肾冲击 10 0 0次 ,观察大鼠肾组织内皮素 (ET)和尿ET、N 乙酰 葡萄糖苷酶 (NAG)、丙二醛 (MDA)水平和Ccr变化及肾脏组织学和超微结构改变。 结果 治疗组尿NAG、MDA的升高幅度和Ccr降低幅度均明显小于对照组 ,组织损伤程度较轻 ,恢复较快 ;SOD 2万U/kg和 4万U/kg组生化指标和病理改变差别无显著性意义 ;维拉帕米组肾组织和尿ET升高幅度下降 ,联用组效果最好。 结论 钙离子拮抗剂可减轻脂质过氧化作用 ,减少ET释放 ,对ESW肾损伤有显著的保护作用 。

Objective To investigate the protective effects of calcium channel blockade and oxygen free radical catalase on ESWL-induced kidney damage. Methods 120 female uninephrectomized rat models were randomizedly divided into six groups.After verapamil and(or) SOD injection,the kidneys were shocked 1 000 times at 14kV by ESW.Then ET level in kidney tissue,levels of NAG,MDA,ET in urine,variation of creatinine clearance (Ccr) and the kidney histopathological changes were evaluated. Results The variation of NAG and MDA level in urine and the Ccr in the therapeutic groups were significantly lower and the histopathological changes were also much slight than those in the control group. There was no significant difference of the biochemical and pathological changes when the dose of SOD increased from 20 thousand units to 40 thousand units per kg body weight. If verapamil was used, the levels of ET in kidney tissue and in urine both decreased.Combined use of SOD and verapamil can induce better protective effects. Conclusions Calcium channel blokade could decrease not only the degree of lipid overoxidation, but also the level of ET-relieve,so it can induce a marked protective effect on ESW-induced kidney damage and the effect could be much enhanced if combined with SOD.