大熊猫卵泡卵母细胞体外受精的初步研究

Preliminary Study on Fertilization In vitro of Follicular Oocytes in Giant Panda(Ailuropoda melanoleuca

用抽吸法和切割法采集非发情季节病亡大熊猫的卵泡卵母细胞，经体外成熟培养后，用大熊猫冷冻精液进行体外受精。结果表明∶（１）在非繁殖季节，大熊猫卵巢中小腔及大腔卵泡卵母细胞仍较丰富，其大腔卵泡卵母细胞可在体外培养成熟；（２） 大熊猫的卵母细胞平均直径为（１４１±６．７）μｍ ，其胞质富含卵黄颗粒；（３）自配 Ｐａｎｄａ Ｉ Ｉ号获能液（含丙酮酸钠、肾上腺素等）可使大熊猫精子有效获能；（４） 大熊猫卵母细胞体外受精后，经培养可观察到第 ２ 极体，有的受精卵开始发生卵裂。

Follicular oocytes were recovered by aspiration and dissection from a giant panda died of disease during nonbreeding season and fertilized in vitro with frozen semen after maturation in vitro. The results showed that : 1) During nonbreeding season, the giant panda′s ovary was still rich with large and small follicular oocytes, and the large follicular oocytes could be matured in vitro; 2) The mean diameter of the oocytes was (141±6.7)μm and lots of cortical granules contained in the cytoplasm of oocyte ; 3) Panda Ⅱ capacitation medium prepared by the authors was effective for sperm capacitation in giant panda ; 4) After fertilization in vitro, it could be observed that the cultured spermmovium released the second polar body and began to cleave.