β-半乳糖苷酶基因在小鼠乳腺细胞表达的研究

Lacz Gene Expression in Mice Mammary Cells

构建了以乳清酸蛋白（ＷＡＰ）５′区２．６ｋｂ为调控序列，指导β－半乳糖苷酶基因的真核表达质粒，采用直接注射质粒于乳腺的方法在小鼠乳腺中表达出β－半乳糖苷酶活性，证实ＷＡＰ基因调控序列的功能正确性，可以用于转基因动物乳腺表达研究，同时证实该方法可以作为一种暂时性表达载体的验证方法．

WAP gene 5′ control region was cloned.Expression plasmid containing WAP gene 5′ control region that directs βgalactosidase genes was constructed and βgalactosidase activity was expressed in mice mammary gland by injecting plasmid into mammary.The result showed that WAP 5′ region might direct foreign gene expression in mammary gland,and the method of injecting plasmid into mammary gland counld be used to check correctness of expression vector.